https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Protocols for obtaining zygotic and somatic embryos for studying the regulation of early embryo development in the model legume Medicago truncatula https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27039 Medicago truncatula to reach the early cotyledon stage. Here, we address the issue by two approaches. The first one establishes a linkage between embryo development and pod morphology in helping indicate the stage of the zygotic embryo. This is particularly based on the number of pod spirals and development of the spines. An alternative way to complement the in vivo studies is via culturing leaf explants to produce somatic embryos. The medium includes an unusual hormone combination - an auxin (1-naphthaleneacetic acid), a cytokinin (6- benzylaminopurine), abscisic acid and gibberellic acid. The different stages can be discerned growing out of the callus without dissection.]]> Wed 11 Apr 2018 15:45:31 AEST ]]> Peroxisomes contribute to reactive oxygen species homeostasis and cell division induction in Arabidopsis protoplasts https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26381 Wed 11 Apr 2018 11:50:23 AEST ]]> Connecting stress to development in the induction of somatic embryogenesis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:25836 Tue 24 Jan 2017 15:55:41 AEDT ]]>